The Vacuum Mushroom is an item that Fawful gave to Bowser early in the events of Mario and Luigi: Bowser's Inside Story. Bowser ate it thinking that it was a Lucky Mushroom, and it would allow him to destroy his nemisis Mario. However, the mushroom instead caused him to suck up Mario, Luigi, Peach, and others.

At the end of the game, after the final boss Dark Bowser is defeated, the mushroom ceases to exist, and Bowser spits out everyone.